|
|
|
|
|
|
|
|
|
|
|
|
| Navarose Act Gels | |
| The Novarose
cross-linked agarose beads are activated according to the bromohydrine method.This
activation method is based upon well-known chemistry wich allows the coupling to be
performed in aqueous solutions. Novarose Act is supplied as an aqueous suspension. After washing, the gel is immediately ready for use. Since no toxic chemicals are involved and the Novarose products are stable at room temperature, the coupling procedure can, as long as the application allows, easily be performed at the bench at ambient temperature. The cross-linked, spherical Novarose Size Exclusion media are used as starting material for the activated gels. They are available in different particle and pore sizes. Agarose beads are normally too soft for modern high performance methods, but, due to a new, patented cross-linking method,Novarose SE beads are rigid and therefore ideal for all modern chromatography techniques. A spacer of 4 to 16 C-atoms separates the ligand from the Novarose bead. |
Being a cross-linked
agarose matrix,Novarose Act pos- sesses most of the characterisics of an
extraordinary, ideal matrix for affinity chromatography (2).To
make it easy to find the right gel for each application,Novarose Act is
available with three different pore sizes each with two degrees of
substitution Novarose Act is a simple matrix for convenient immobi-
lization of ligands.It is very easy to use and offers high flexibility and high
performance.The gels can be stored at room temperature for one year in aqueous solution at
pH 7 (containing ~20 %methanol as preservative)without any significant decrease in
coupling activity. Application Areas Novarose™ Act High and Novarose™ Act Low are tailor-made for purification of biomolecules using different chromatographic techniques – IEX, HIC, IMAC or affinity chromatography – after coupling with a suitable ligand. As mentioned, they are the perfect gels for easy and reliable immobilization of ligands containing sulfydryl, amino or hydroxyl groups. |
