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Toyopearl HW size exclusion chromatography (SEC) resins are macroporous
packings dedicated to the separation of large biopolymers such as proteins, nucleic acids
etc.They are also appropriate for molecular weight determination.These resins are
semi-rigid, spherical beads with a hydrophilic surface.They are synthezised by
co-polymerisation of ethylene glycol and methacrylate-type polymers.There are five types
of Toyopearl HW resins available, i.e., HW-40, HW-50, HW-55, HW-65 and HW-75. As easily
can be concluded from figure and table, each of these is defined by a given molecular size
separation range.They span peptide and protein molecular weights, from 100 to 50,000,000
Daltons depending on resin type. Separation
of ß-cyclodextrin hydrolysate |
