| The numerous surface hydroxyl groups of the Toyopearl HW-55 and HW-65
resins, which have been well-proven in size exclusion chromatography, provide
excellent attachment points for other functional groups and ligands.Thus, these resins are
used as the principal base matrix for the Toyopearl 550 and 650 series of
ion exchange resins.The Toyopearl 550 series of IEC resins is designed to enable high
volume sample throughput, whereas Toyopearl 650 series provides the high resolution
required during the mid to final stages of a purification process. They have an estimated
globular protein exclusion limit of 700000 and 5000000 Daltons respectively. All Toyopearl
ion exchangers are chemically and thermally stable due to their polymeric backbone. They
can be used from pH 3.0 to pH 10.5. Caustic or acidic solutions can also be applied for
sanitation (e.g.,1 %sodium hypochlorite) and depyrogenation. Furthermore, in some cases,
overnight cleaning or sterilisation procedures with strong acid or base are acceptable,
for instance with Toyopearl DEAE-650. Comparison of resolution by strong cation exchangers |
 |
