TSK-Gel

TSK-GEL 5PW Preparative LC Resins
packed in NovoGROM columns

The ultimate alternative to preparative HPLC

TSK-GEL 5PW Preparative LC Resins are most often used for up-scaling in biopharmaceutical applications.The TSKgel DEAE-5PW and TSK-gel SP-5PW resins permit high resolution in ion exchange chromatography, whereas the outstanding, TSKgel Phenyl-5PW and TSKgel Ether-5PW resins are used for up-scaling in hydrophobic interaction chromatography. They have a particle size of 30 痠. All are made from the same hydrophilic G5000 PW-polymer and guarantee excellent preparative separations, especially when packed in the unique NovoGROM columns.

The huge variety of NovoGROM HPLC columns is offered packed with

Resin-based TSK-GEL reversed phases

They provide maximum selectivity and resolution for a wide range of peptides and proteins.The hydrophilic backbone of these TSKgel reversed phase packings guarantees unsurpassed chemical and physical stability for longer column life. Several problems inherent to silica based packings, such as a lack of pH stability or secondary interactions due to exposed silanol groups, are eliminated when columns are packed with TSKgel reversed phase resins.

Rapid peptide separation on TSK-gel Octadecyl-NPR
Experimental conditions: column: TSK-gel Octadecyl-NPR, 4.6 x 35 mm; eluent / graduent: 10 min linear from 0 % to 80 % ACN in 0.2 % TFA; flow rate: 1.5 ml /min; temperature: ambient; detection: UV, 220 nm; sample: 1) a-endorphin, 2) bombesin, 3) y-endorphin, 4) angiotensin, 5) somatostatin, 6) calcitonin