Why use short(er) columns? ... |
| ... High Speed HPLC with short columns offers numerous advantages and benefits: |
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short analysis time - only 1/7 of standard HPLC |  |
high sample through-put, i.e. tremendously reduced costs per analysis |
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low solvent consumption - only 1/5 of standard HPLC | |
cost saving + improved environmental compatibility |
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decreased diffusion /dispersion | |
increased sensitivity - > 2-fold - |
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increased performance, i.e. minimum band broadening- | |
higher number (N) of hight equivalents of theoretical plates (HETP) per meter - > 4.5-fold - |
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| Stationary phase: GROM SIL 100 ODS-2 FE; Linear velocity: 0.8 mm/s; Eluent: ACN:H2O = *65:35, resp. **60:40; Flow cell: 1.2 µl / 3 mm with quick-connector (100 x 0.1 mm capillary); Injection: 5 µl benzoate test mixture / 1:100 dil. (methyl-, ethyl-, propyl-, butyl-, pentyl benzoate; 10-20 mg/ml) |
| Varying column length and particle size can substantially reduce runtimes and solvent consumption without compromising sensitivity or resolution |
| Column Length Particle Size |
AnalysisTime [min] |
Solvent
Consumption [ml] |
Sensitivity
(1st peak ) [AU] |
Resolution
(1. + 2nd peak) |
N/m (5th peak ) |
| 250 x 4 mm * 10 µm |
18.1 | 10.9 | 0.004 | 4.8 | 29 000 |
| 125 x 4 mm * 5 µm |
9.4 | 5.6 | 0.008 | 5.2 | 72 200 |
| 60 x 4 mm ** 3 µm |
4.8 | 2.9 | 0.013 | 4.6 | 129 300 |
| 33 x 4.6 mm * 1.5 µm |
2.5 | 2.0 | 0.010 | 2.5 | 130 900 |