Novel High Performance High Speed Liquid Chromatography

       

Button_mini_pink.gif (1036 Byte) for price informations and order numbers of High Speed HPLC Columns

        
either by uniformly spherical, porous 1.5 m ODS particles or by fully spherical, non-porous 1.5 m ODS particles packed in the unique, out-standing NovoGROM 33 x 2.0 mm or resp. 33 x 4.6 mm column hardware
10 179 Peptides, tryptic digested of lactic dehydrogenase (carboxymethylated)
For high-speed, high-resolution HPLC two types of uniformly spherical 1,5 m GROM-Sil ODS-2 FE particles are available, either fully porous microspheres (pore size 100 /particle size 1.5 0.3 m, specific surface area of 200 m 2 /g) or monodisperse non-porous beads (particle size 1.5 0.05 m, specific surface area of 2.1 m 2 /g). Both are fully end-capped C18 phases meeting all the requirements of the most advanced high performance liquid chromatography applications. For instance, in a minimum of analysis time, they perform excellent separations of nucleotides, peptides, proteins, pharmaceuticals, etc. 10179 Seite.gif (14479 Byte)
Column phase:
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GROM-SIL 100 ODS-2 FE,1.5 m
33 x 4.6 mm
H2O, 0.1% TFA
ACN, 0.1% TFA
0 -15 min, 5 - 60% B
1ml / min
215 nm
RT
2 l
Even after more than 2 500 injections,both types of columns packed with either 1.5 m porous-or 1.5 m non-porous particles still show excellent physical and chemical stability (even in 0.1%TFA containing eluents).There is no change in retention time and practically no increase in pressure drop and peak asymmetry over the life of the column. These NovoGROM high speed columns of only 33 mm length provide up to 10 000 plates,and thus in most cases have a resolution nearly identical to that which is typically found for standard analytical HPLC columns. columnlife.gif (8322 Byte)

 Column lifetime

Employing NovoGROM high speed columns you gain tremendously in
• Speed: analysis time in seconds or minutes, with up to 90%increase in sample throughput
• Low solvents consumption: saves on purchase and disposal costs, decreasing solvent consumption brings improved environmental compatibility
• Decreased diffusion: smaller peak volume and less band broadening, i.e.,improved sensitivity

 

10 030 b Sedatives -Benzodiazepins
10 031b Nonsteroid anti-inflammatory drugs
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Column phase:
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Sample:
GROM-SIL 100 ODS-2 FE, 1.5 m
33 x 4.6 mm
35%v/v Acetonitrile, 65%v/v 0.1%TFA
1.5 ml/min
26.2 MPa
RT
254 nm
1 l
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Sample:
GROM-SIL 100 ODS-2 FE, 1.5 m
33 x 4.6 mm
40%v/v Acetonitrile, 60%v/v 0.1%TFA
1.5 ml/min
26 MPa
RT
254 nm
1 l (100 g/ml, Ibuprofen 1000 g/ml)
10 032 c Analgesic, antipyretic Thomapyrin pill
10 053b High Speed HPLC Analysis -Determination
of Optical Antipodes of a -Blocker
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Column phase:
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Sample:
GROM-SIL 100 ODS-2 FE, 1.5 m
33 x 4.6 mm
15%v/v  Acetonitrile,  85%v/v  0.1% TFA
1.5 ml/min
29.2 MPa
RT
274 nm; after 1 min, 227 nm
10 l (Paracetamol 2 mg/m 2, Coffeine 0.5 mg/ml,
ASA 2 mg/ml)
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Injection:
GROM-SIL 100 ODS-2 FE,1.5 m
33 x 4.6 mm
water /acetonitrile =30 / 70
1.0 ml/min
18 MPa
RT
263 nm (exc.), 313 nm (em.)
1 l (=500 fmol)

 

10 180 Nucleotides
10 182 Protein Digest
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Column phase:
Column size:
Eluent A:
B:
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Detection
(UV):
Sample:
GROM-SIL 100 ODS-2 FE, 1.5 m
33 x 4.6 mm
A:30 mM KH 2 PO 4 /K 2 HPO 4 , 0.5 MTBA, pH 6.0
60%A + 40% ACN
0 - 40%B (0 - 8 min)
1.5 ml/min
RT
254 nm
10 l (10 g each)
Column phase:
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(UV):
Sample:
GROM-SIL 100 ODS-2 FE, 1.5 m
33 x 4.6 mm
0.05 M TEAA, pH 7.0
30% A +70% ACN
0 – 70% B (0 – 6 min)
1.0 ml/min
RT
260 nm
10 l (10 g each)
10 181 Antisense-Oligonucleotides
10 162 High Speed Separation of Benzoates
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Column phase:
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Eluent A:
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Detection (UV):
Sample:
GROM-SIL 100 ODS-2 FE, 1.5 m
33 x 2.0 mm
0.1% TFA in H2O
0.1% TFA in ACN
5 - 0% B (0 - 12 min)
0.2 ml/min
10 - 27 MPa
RT
214 nm
1 l (5 pMol)
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Sample:

GROM-SIL-ODS 0 AB, 1.5 m
33 x 4 mm
H2O / ACN =30 / 70
2 ml/min
23,8 MPa
RT
254 nm
1 l (0,5 - 1,5 mg/ml of each)