high speed liquid

Novel High Performance High Speed Liquid Chromatography

for price informations and order numbers of High Speed HPLC Columns

either by uniformly spherical, porous 1.5 µm ODS particles or by fully spherical, non-porous 1.5 µm ODS particles packed in the unique, out-standing NovoGROM 33 x 2.0 mm or resp. 33 x 4.6 mm column hardware

10 179

Peptides, tryptic digested of lactic dehydrogenase (carboxymethylated)

For high-speed, high-resolution HPLC two types of uniformly spherical 1,5 µm GROM-Sil ODS-2 FE particles are available, either fully porous microspheres (pore size 100 Å /particle size 1.5 ± 0.3 µm, specific surface area of 200 m 2 /g) or monodisperse non-porous beads (particle size 1.5 ± 0.05 µm, specific surface area of 2.1 m 2 /g). Both are fully end-capped C18 phases meeting all the requirements of the most advanced high performance liquid chromatography applications. For instance, in a minimum of analysis time, they perform excellent separations of nucleotides, peptides, proteins, pharmaceuticals, etc.

Column phase:
Column size:
Eluent A:
Eluent B:
Gradient:
Flow rate:
Detection(UV):
Temperature:
Injection:

GROM-SIL 100 ODS-2 FE,1.5 µm
33 x 4.6 mm
H₂O, 0.1% TFA
ACN, 0.1% TFA
0 -15 min, 5 - 60% B
1ml / min
215 nm
RT
2 µl

Even after more than 2 500 injections,both types of columns packed with either 1.5 µm porous-or 1.5 µm non-porous particles still show excellent physical and chemical stability (even in 0.1%TFA containing eluents).There is no change in retention time and practically no increase in pressure drop and peak asymmetry over the life of the column. These NovoGROM high speed columns of only 33 mm length provide up to 10 000 plates,and thus in most cases have a resolution nearly identical to that which is typically found for standard analytical HPLC columns.

	Column lifetime
Employing NovoGROM high speed columns you gain tremendously in
• Speed:	analysis time in seconds or minutes, with up to 90%increase in sample throughput
• Low solvents consumption:	saves on purchase and disposal costs, decreasing solvent consumption brings improved environmental compatibility
• Decreased diffusion:	smaller peak volume and less band broadening, i.e.,improved sensitivity

10 030 b

Sedatives -Benzodiazepins

10 031b

Nonsteroid anti-inflammatory drugs


Column phase: Column size: Eluent: Flow rate: Pressure: Temperature: Detection: Sample:	GROM-SIL 100 ODS-2 FE, 1.5 µm 33 x 4.6 mm 35%v/v Acetonitrile, 65%v/v 0.1%TFA 1.5 ml/min 26.2 MPa RT 254 nm 1 µl	Column phase: Column size: Eluent: Flow rate: Pressure: Temperature: Detection: Sample:	GROM-SIL 100 ODS-2 FE, 1.5 µm 33 x 4.6 mm 40%v/v Acetonitrile, 60%v/v 0.1%TFA 1.5 ml/min 26 MPa RT 254 nm 1 µl (100 µg/ml, Ibuprofen 1000 µg/ml)

10 032 c

Analgesic, antipyretic Thomapyrin pill

10 053b

High Speed HPLC Analysis -Determination
of Optical Antipodes of a ß -Blocker

10053b Seite.gif (6186 Byte)


Column phase: Column size: Eluent: Flow rate: Pressure: Temperature: Detection: Sample:	GROM-SIL 100 ODS-2 FE, 1.5 µm 33 x 4.6 mm 15%v/v Acetonitrile, 85%v/v 0.1% TFA 1.5 ml/min 29.2 MPa RT 274 nm; after 1 min, 227 nm 10 µl (Paracetamol 2 mg/m 2, Coffeine 0.5 mg/ml, ASA 2 mg/ml)	Column phase: Column size: Eluent: Flow rate: Pressure: Temperature: Detection (Fluor.): Injection:	GROM-SIL 100 ODS-2 FE,1.5 µm 33 x 4.6 mm water /acetonitrile =30 / 70 1.0 ml/min 18 MPa RT 263 nm (exc.), 313 nm (em.) 1 µl (=500 fmol)

10 180

Nucleotides

10 182

Protein Digest


Column phase: Column size: Eluent A: B: Gradient: Flow rate: Temperature: Detection (UV): Sample:	GROM-SIL 100 ODS-2 FE, 1.5 µm 33 x 4.6 mm A:30 mM KH 2 PO 4 /K 2 HPO 4 , 0.5 MTBA, pH 6.0 60%A + 40% ACN 0 - 40%B (0 - 8 min) 1.5 ml/min RT 254 nm 10 µl (10 µg each)	Column phase: Column size: Eluent A: B: Gradient: Flow rate: Temperature: Detection (UV): Sample:	GROM-SIL 100 ODS-2 FE, 1.5 µm 33 x 4.6 mm 0.05 M TEAA, pH 7.0 30% A +70% ACN 0 – 70% B (0 – 6 min) 1.0 ml/min RT 260 nm 10 µl (10 µg each)

10 181

Antisense-Oligonucleotides

10 162

High Speed Separation of Benzoates


Column phase: Column size: Eluent A: B: Gradient: Flow rate: Pressure: Temperature: Detection (UV): Sample:	GROM-SIL 100 ODS-2 FE, 1.5 µm 33 x 2.0 mm 0.1% TFA in H₂O 0.1% TFA in ACN 5 - 0% B (0 - 12 min) 0.2 ml/min 10 - 27 MPa RT 214 nm 1 µl (5 pMol)	Column phase: Column size: Eluent: Flow rate: Pressure: Temperature: Detection (UV.): Sample:	GROM-SIL-ODS 0 AB, 1.5 µm 33 x 4 mm H₂O / ACN =30 / 70 2 ml/min 23,8 MPa RT 254 nm 1 µl (0,5 - 1,5 mg/ml of each)