Column-sets for the development of chromatographic methods

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Button_mini_gruen.gif (1036 Byte)Method Development Sets at a glance: 9 different Microbore MD-Sets and 8 analytical MD-Sets
Button_mini_gruen.gif (1036 Byte)UP-Scaling - Column Set

Cost-effective method development: saves both time and solvent consumption

For the development of new analytical methods, in particular for the selection of the appropriate stationary phase in a minimum of time and with extremely low solvent consumption, GROM offers its new Method-Development-sets.These consist of five cartridges packed with stationary phases of differing selectivity.

The use of short columns (60mm) and small particles (generally 3 µm, in a few cases 5 µm) results in significant savings of both time and solvent consumption in the process of optimising the various parameters of separation such as flow rate, eluent composition, temperature, gradient shape, etc.MD-Sets are available as cost-effective cartridges of 60 mm length and 2 or 4 mm internal diameter and can be operated with either standard column ends or with the NovoGROM quick connectors (see pages 107 and 113). With the microbore version (2 mm i.d.), the expenditure for solvents (purchase and disposal)is around 75%lower and the sensitivity approximately 4 times higher than with conventional 4 mm i.d.columns.

  The combination of an MD-Set with an autoinjector and automatic column switching represents a highly convenient and elegant system for HPLC-method development. In order to guarantee highest quality, each column of the MD-Set is individually tested. The resolution attained not only allows effective screening of the experimental conditions, but often also fulfills many analytical requirements such as in product quality control or the monitoring of large-scale syntheses.

 

 
 
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Method-Development-Sets
Composed of five cartridges (60 x 4 mm or 60 x 2 mm) filled with stationary phases of differing selectivity (without column ends and without quick connectors). The particle diameter is generally 3 µm.

 

NovoGROM Method Development Sets at a glance:
9 different Microbore MD-Sets and 8 analytical MD-Sets		 Pfeil.gif (145 Byte) back to the top
   
MD-Set for„Peptides"    MD-Set for „Peptides"   MD-Set for „Standard Rev. Phases"
-decreasing hydrophobicity
     
GROM-SIL 100 ODS-2 FE, 3 µm
GROM-SIL 120 ODS-4 HE, 3 µm
GROM-SIL 100 Octyl-2 AB, 3 µm
GROM-SIL 120 Phenyl-2 CP, 5 µm
GROM-SIL 120 Butyl-1 ST, 3 µm
   
    
GROM-SIL 300 Octyl-5 CP, 5 µm
GROM-SIL 300 Phenyl-2 CP, 5 µm
GROM-SIL 300 Butyl-2 FE, 3 µm
GROM-SIL 300 SA-1, 3 µm
GROM-SIL 300 WC-2 7 µm
   
       
GROM-SIL 080 ODS-7 pH, 4 µm
GROM-SIL 100 ODS-0 AB, 3 µm
GROM-SIL 100 Octyl-4 FE, 3 µm
GROM-SIL 120 Butyl-1 ST, 3 µm
GROM-SIL 120 TMS-2 CP, 5 µm
     
Order-Nr. Order-Nr.   Order-Nr.
GS ME1 0312 K 0602, resp. 04
(2 mm and 4 mm cartridges, respectively)		 GS ME2 0530 K 0602, resp. 04
(2 mm and 4 mm cartridges, respectively)		 GS ME3 0310 K 0602, resp. 04
(2 mm and 4 mm cartridges,respectively)
     
MD-Set for „Normal-Phase /
Reversed Phase"		 MD-Set for „C 18-Phases"
-with differing pore sizes-		 MD-Set for „C18-Phases"
-with differing C-content-
    
GROM-SIL 100 Amino-1 PR, 5 µm
GROM-SIL 100 Cyan-2 PR, 3 µm
GROM-SIL 120 Phenyl-2 CP, 5 µm
GROM-SIL 120 TMS-2 CP, 5 µm
GROM-SIL 100 Normal Ph-1, 3 µm
         
    
SIL 080 ODS-2 FE, 3 µm
GROM-SIL 120 ODS-3 CP, 3 µm
GROM-SIL 200 ODS-5 ST, 3 µm
GROM-SIL 300 ODS-2 FE, 3 µm
GROM-SIL 500 ODS-2 FE, 5 µm
          
 
    
SIL 100 ODS-1 PE, 3 µm
GROM-SIL 100 ODS-2 FE, 3 µm
GROM-SIL 120 ODS-3 CP, 3 µm
GROM-SIL 120 ODS-5 ST, 3 µm
GROM-SIL 080 ODS-7 pH, 4 µm
   
Order-Nr. Order-Nr. Order-Nr.
GS ME4 0510 K 0602, resp. 04
(2 mm and 4 mm cartridges, respectively)		 GS ME5 0399 K 0602, resp. 04
(2 mm and 4 mm cartridges, respectively)		 GS ME6 0312 K 0602, resp. 04
(2 mm and 4 mm cartridges,respectively)
        
MD-Set for
„Basic Compounds"		 MD-Set for
„Chiral Separations"
-in ‘normal’ or ‘reversed’ mode-		 MD-Set for
„Free Choice"
-custom assortment-
    
GROM-SIL 100 ODS-0 AB, 3 µm
GROM-SIL 120 ODS-3 CP, 3 µm
GROM-SIL 100 Octyl-2 AB, 3 µm
GROM-SIL 120 Octyl-3 BA, 3 µm
GROM-SIL 120 Phenyl-2 CP, 5 µm
       
     
CHIRA-GROM 1, 8 µm
CHIRA-GROM 2, 8 µm
CHIRA-GROM 3, 8 µm
CHIRA-GROM 4, 8 µm
CHIRA-GROM 9, 8 µm
     

consisting of 5 MD-cartridges with different, customer specified GROM SIL phases (except chiral phases)

Please contact us for help!
Order-Nr. Order-Nr. Order-Nr.
GS ME7 0312 K 0602, resp. 04
(2 mm and 4 mm cartridges, respectively)		 GS ME8 0891 K 06 02
(2 mm cartridges)		 GS ME 9 0000 K 06 02, resp. 04
(2 mm and 4 mm cartridges,respectively)
   

Note!

Method-Development means in German „Methoden-Entwicklung"; therefore order numbers are always GS ME

 

NovoGROM „Up-scaling"- Column Set Pfeil.gif (145 Byte) back to the top
   
For the scale-up of optimized analytical separations without unnecessary waste of expensive samples and solvents, GROM offers its „Up-scaling" Sets.
These consist of an analytical column (250 x 4 mm) and a preparative column (either 250 x 20 mm or 250 x 40 mm), both of which are filled with completely identical packing (i.e.from the same batch).

This means:
– identical particle diameter,
– identical pore radius,
– identical coating,
– and, most important, the same silica
gel as starting material, guaranteeing identical selectivity of both columns.

A separation optimized on the analytical column (with savings of solvents -both purchase, preparation and disposal) will yield identical resolution when transfered under identical operating parameters (temperature, linear flow rate*, etc.) to the preparative column.

UP-sets thus help to avoid troublesome, time consuming experiments in transferring an analytical separation to a preparative scale.
This is particularly true when the analytical separation has been performed on a column filled with 3 µm diameter particles whereas the preparative column is packed with cheaper, coarser (e.g.,15 µm) particles, thus resulting in different selectivities of the two columns.

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UP-scaling-set with 250 x 4 mm analytical column and 250 x 20 mm preparative column
 
UP-scaling-sets, consisting of
one analytical column -250 x 4 mm
one preparative column -250 x 20 mm (or 40 mm, respectively)
and with the following packings are available from stock:
   
GROM-SIL 120 ODS-4 HE,10 µm Order No.GS OD4 1112 U 25 20
Resp.GS OD4 1112 U 25 40
GROM Sapphire 110 C8,10 µm Order No.GS OCS 1011 U 25 20
Resp.GS OCS 1011 U 25 40
GROM-SIL 120 Si NP-2,10 µm Order No.GS NP2 1112 U 25 20
Resp.GS NP2 1112 U 25 40
   
    UP-sets with other stationary phases or with columns of other dimensions upon request.
   
 

*For constant linear flowrate:
volumetric flowrate of the prep. column = (vol.flow rate of the analytical column /cross-section of the anal.column) x cross-section of the prep.column.

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  UP-set with 250 x 4 mm analytical column and 250 x 40 mm preparative column.