| CHIRA-GROM Phases for the separation of chiral molecules by High Performance Liquid Chromatography | ||
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| I. NovoGROM column hardware for chiral separations | ||
| In order to increase sensitivity, to decrease costs by saving solvents and
for environmental protection tailor-made, customized CHIRA-GROM matrices are packed in the low dispersion NovoGROM microbore columns. This well-designed, excellent column hardware performs
outstandingly in all, even highly sophisticated, chiral separations. There are three versions of microbore columns packed with CHIRA-GROM-phases available:• Standard microbore column (250 x 2 mm) - connected to the HPLC-system via 1/16" capillaries, ferrules and compression screws • Low dispersion microbore cartridge (250 x 2 mm) - with endfittting adapters and column heads (quick-connectors) • Microbore guard column combination (250 x 2 mm column and 10 x 2 mm guard column) - with endfittting adapters, spacer and guard column head or as a novel alternative: CHIRA-GROM phases packed in the unique, NovoGROM capillary columns, with fingerthight fittingsStandard capillary column - length 250 mm and 200, 300, 500 or 800 µm i.d. Capillary column (length 250 mm) with integrated guard column - (lenght 5 or 20 mm) both columns with 200, 300, 500 or 800 µm i.d. |
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| II. Five different CHIRA-GROM matrices are available: | ||
CHIRA-GROM Phases Type 1-4 and 9
consist of spherical, highly porous 8 µm silica particles specially treated by a novel thermal process and
covered with layers of synthetic chiral polymers. They are stable up to 35 MPa and may be
used from pH 1 to 8 at temperatures of 0-80°C. With these high-performance materials,
separations of chiral molecules can easily be achieved in the normal phase, as well in the
reversed phase mode. For NovoGROM Method Development Sets for chiral separations follow
link .There are five major advantages of working in the reversed phase mode, i.e., using polar solvents to separate chiral molecules: |
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| 1) | After sample dilution, crude biological samples from conventional matrices can be applied directly, for instance, by column switching. | |
| 2) | Up to now, polar chiral molecules insoluble in hydrophobic phases such as heptane etc. could not be separated.However, now they can be dissolved in polar mobile phases and therefore are easily separated in the reversed phase mode. | |
| 3) | Gradient elution is simple in the reversed phase mode. | |
| 4) | For many separations, better values of "a", i. e., relative retention may be achieved. | |
| 5) | Column lifetime is generally superior. | |
| Typical fields of application are:• purity check of chiral samples (pharmaceuticals, agrochemicals,
flavorings) • chiral drug monitoring of biological materials: plasma, urine, tissues, etc. • LC-MS coupling |
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